For lack of a better choice, the same technology has been adopted for localization microscopy. Our “quiet” electronics successfully lower the limit of detection, allowing you to take full advantage of high frame rates and see your signal with fewer photons. With CCDs there are never any issues regarding which model to use because the typical read noise for all pixels is very similar; thus rms and median are equivalent.
New in the ORCA-Flash4.0 V2 is an additional slow scan readout mode with read noise of just 1.4 electrons rms (0.8 electrons median). Both the USB and Camera Link configurations of the camera have this low noise capability. With the ORCA-Flash4.0 V2 already delivering wide field of view, large dynamic range, and fast frame rates, this QE enhancement only makes it more versatile and powerful.If you have not yet experienced the ORCA-Flash4.0 V2 sCMOS, now is the time. With sCMOS, the structure of the sensor inherently has more pixel variation and the extreme low noise of the sensor makes variation more statistically significant. This enhanced QE means that you have a possibility of detecting the faintest of signals. Or, for moderately dim samples, that the ideal image is achievable with shorter exposure times, perhaps saving your cells from phototoxicity or bleaching.